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| Corrêa-Oliveira,R.; Malaquias,L.C.C.; Falcão,P.L.; Viana,I.R.C.; Bahia-Oliveira,L.M.G.; Silveira,A.M.S.; Fraga,L.A.O.; Prata,A.; Coffman,R.L.; Lambertucci,J.R.; Cunha-Melo,J.R.; Martins-Filho,O.A.; Wilson,R.A.; Gazzinelli,G.. |
| The role of different cytokines in the peripheral blood mononuclear cell (PBMC) proliferative response and in in vitro granuloma formation was evaluated in a cross-sectional study with patients with the different clinical forms and phases of Schistosoma mansoni infection, as well as a group of individuals "naturally" resistant to infection named normal endemic (NE). The blockage of IL-4 and IL-5 using anti-IL-4 and anti-IL-5 antibodies significantly reduced the PBMC proliferative response to soluble egg (SEA) and adult worm (SWAP) antigens in acute (ACT), chronic intestinal (INT) and hepatosplenic (HS) patients. Similar results were obtained in the in vitro granuloma formation. Blockage of IL-10 had no significant effect on either assay using PBMC from ACT... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Schistosoma mansoni human schistosomiasis mansoni cytokines IL-10 IL-4 IFN-<; FONT FACE=Symbol>; G<; /font>. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100024 |
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| Brito,C.F.A.; Oliveira,G.C.; Oliveira,S.C.; Street,M.; Riengrojpitak,S.; Wilson,R.A.; Simpson,A.J.G.; Correa-Oliveira,R.. |
| Sm14 is a 14-kDa vaccine candidate antigen from Schistosoma mansoni that seems to be involved in cytoplasmic trafficking of fatty acids. Although schistosomes have a high requirement for lipids, they are not able to synthesize fatty acids and sterols de novo. Thus, they must acquire host lipids. In order to determine whether Sm14 is present in different stages of the life cycle of the parasite, we performed RT-PCR. Sm14 mRNA was identified in all stages of the life cycle studied, mainly schistosomulum, adult worm and egg. Additionally, we used a rabbit anti-Sm14 polyclonal antibody in an indirect immunofluorescence assay to localize Sm14 in adult worm sections. The basal lamella of the tegument and the gut epithelium were strongly labeled. These tissues... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Sm14; Fatty acid-binding protein; FABP; Immunolocalization; RT-PCR. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300014 |
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